Fig. 1. AQP2 and CHIP co-immunoprecipitation in MCD4 cells and renal tissue. AQP2 was immunoprecipitated (I.P. AQP2) using pre-C-tail antibodies from MCD4 cells (A) and from fresh mouse kidney slices (B). Whole cell lysates were also subjected to precipitation with only protein A-coupled Sepharose (ProtA) or with nonspecific IgG. Immunocomplexes were analyzed by immunoblotting with antibodies specific for CHIP; anti-AQP2 antibodies were used as control of the immunoprecipitation experiments. The obtained results indicate that AQP2 can complex with CHIP in MCD4 cells and in renal tissue.